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Induction of PSA-specific CTLs and anti-tumor immunity by a genetic prostate cancer vaccine

Item Type:Article
Title:Induction of PSA-specific CTLs and anti-tumor immunity by a genetic prostate cancer vaccine
Creators Name:Roos, A.K., Pavlenko, M., Charo, J., Egevad, L. and Pisa, P.
Abstract:BACKGROUND. Prostate cancer is the most common malignancy in Swedish and American men. Effective curative treatment modalities are debilitating and available only for localized disease. As an immunotherapy approach, DNA encoding prostate-specific antigen (PSA), was used to immunize mice and induce PSA-specific cellular immunity. METHODS. A plasmid expressing PSA, alone or in combination with plasmids coding for GM-CSF and/or IL-2, was used for DNA immunization. Cr-release, intracellular IFN-γ cytokine staining, and tumor challenge assays were used to evaluate the immune response. RESULTS. The DNA vaccine induces PSA-specific cytotoxic T lymphocytes (CTLs) and when co-injected with IL-2 and GM-CSF it protects four of five mice against a PSA-expressing tumor challenge. CONCLUSIONS. We demonstrate that immunization with a PSA DNA vaccine can evoke PSA-specific cellular immune responses. We also show, for the first time, that a PSA DNA vaccine can induce anti-tumor immunity in vivo.
Keywords:CTL, DNA Vaccine, IFN-γ, Immunotherapy, Prostate-specific antigen, Animals, Mice
Page Range:217-223
Date:1 January 2005
Official Publication:https://doi.org/10.1002/pros.20135
PubMed:View item in PubMed

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