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| Item Type: | Article |
|---|---|
| Title: | Proliferation of human melanoma cells is under tight control of protein kinase C alpha |
| Creators Name: | Krasagakis, K., Lindschau, C., Fimmel, S., Eberle, J., Quass, P., Haller, H. and Orfanos, C.E. |
| Abstract: | Exponential proliferation of human melanoma cells has been associated with low levels of protein kinase C (PKC)-{alpha}. The aim of the present study was to investigate the functional relationship between PKC-{alpha} and melanoma cell proliferation. Treatment of human melanoma cells with the selective PKC inhibitor Ro-31-8220 resulted in a significant increase of cell proliferation as measured by (3)H-thymidine incorporation and a fluorometric microassay. In addition, phosphorothioate antisense-oligodeoxynucleotides (ODNs) to PKC-{alpha} enhanced DNA-synthesis of human melanoma cells. Furthermore, microinjection and transient transfection of melanoma cells with PKC-{alpha} decreased their proliferation, as shown by the reduction of nuclear staining with the proliferation marker Ki-67. The presented data demonstrate a cause-effect relationship between PKC-{alpha} and melanoma cell growth, whereby PKC-{alpha} reversely influences the rate of cell proliferation. |
| Keywords: | Antisense Oligodeoxyribonucleotides , Cell Division, Confocal Microscopy, Enzyme Inhibitors, Fluorescent Antibody Technique, Indoles, Melanoma, Microinjections, Protein Kinase C, Protein Kinase C-alpha, Transfection, Tumor Cell Line |
| Source: | Journal of Cellular Physiology |
| ISSN: | 0021-9541 |
| Publisher: | Wiley-Blackwell |
| Volume: | 199 |
| Number: | 3 |
| Page Range: | 381-387 |
| Date: | June 2004 |
| Official Publication: | https://doi.org/10.1002/jcp.10434 |
| PubMed: | View item in PubMed |
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