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| Item Type: | Article |
|---|---|
| Title: | Sulfur oxidation in Paracoccus pantotrophus: interaction of the sulfur-binding protein SoxYZ with the dimanganese SoxB protein |
| Creators Name: | Quentmeier, A., Hellwig, P., Bardischewsky, F., Grelle, G., Kraft, R. and Friedrich, C.G. |
| Abstract: | The central protein of the sulfur-oxidizing enzyme system of Paracoccus pantotrophus, SoxYZ, formed complexes with subunits associated and covalently bound. In denaturing SDS-polyacrylamide gel electrophoresis (PAGE) SoxY migrated at 12 and SoxZ at 16kDa. SDS-PAGE of homogeneous SoxYZ without reductant separated dimeric complexes of 25, 29, and 32kDa identified by the N-terminal amino acid sequences as SoxY-Y, SoxY-Z, and SoxZ-Z, and subunit cleavage by reduction suggested their linkage via protein disulfide bonds. SoxYZ was reversibly redox active between -0.25 and 0.2V, as monitored by a combined electrochemical and FTIR spectroscopic approach. The dimanganese SoxB protein (58.611Da) converted the covalently linked heterodimer SoxY-Z to SoxYZ with associated subunits which in turn aggregated to the heterotetramer Sox(YZ)2. This reaction depended on time and the SoxB concentration, and demonstrated the interaction of these two Sox proteins. |
| Keywords: | FTIR Spectroscopy, Paracoccus Pantrotrophus, Protein Disulfide, Redox Properties, SoxYZ Protein |
| Source: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Publisher: | Academic Press |
| Volume: | 312 |
| Number: | 4 |
| Page Range: | 1011-1018 |
| Date: | 1 January 2003 |
| Official Publication: | https://doi.org/10.1016/j.bbrc.2003.11.021 |
| PubMed: | View item in PubMed |
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