The genome sequence of the malaria mosquito Anopheles gambiae

Tools

Item Type:	Article
Title:	The genome sequence of the malaria mosquito Anopheles gambiae
Creators Name:	Holt, R.A., Subramanian, G.M., Halpern, A., Sutton, G.G., Charlab, R., Nusskern, D.R., Wincker, P., Clark, A.G., Ribeiro, J.M.C., Wides, R., Salzberg, S.L., Loftus, B., Yandell, M., Majoros, W.H., Rusch, D.B., Lai, Z.W., Kraft, C.L., Abril, J.F., Anthouard, V., Arensburger, P., Atkinson, P.W., Baden, H., de Berardinis, V., Baldwin, D., Benes, V., Biedler, J., Blass, C., Bolanos, R., Boscus, D., Barnstead, M., Cai, S., Center, A., Chatuverdi, K., Christophides, G.K., Chrystal, M.A., Clamp, M., Cravchik, A., Curwen, V., Dana, A., Delcher, A., Dew, I., Evans, C.A., Flanigan, M., Grundschober-Freimoser, A., Friedli, L., Gu, Z.P., Guan, P., Guigo, R., Hillenmeyer, M.E., Hladun, S.L., Hogan, J.R., Hong, Y.S., Hoover, J., Jaillon, O., Ke, Z.X., Kodira, C., Kokoza, E., Koutsos, A., Letunic, I., Levitsky, A., Liang, Y., Lin, J.J., Lobo, N.F., Lopez, J.R., Malek, J.A., McIntosh, T.C., Meister, S., Miller, J., Mobarry, C., Mongin, E., Murphy, S.D., O'Brochta, D.A., Pfannkoch, C., Qi, R., Regier, M.A., Remington, K., Shao, H.G., Sharakhova, M.V., Sitter, C.D., Shetty, J., Smith, T.J., Strong, R., Sun, J.T., Thomasova, D., Ton, L.Q., Topalis, P., Tu, Z.J., Unger, M.F., Walenz, B., Wang, A.H., Wang, J., Wang, M., Wang, X.L., Woodford, K.J., Wortman, J.R., Wu, M., Yao, A., Zdobnov, E.M., Zhang, H.Y., Zhao, Q., Zhao, S.Y., Zhu, S.P.C., Zhimulev, I., Coluzzi, M., della Torre, A., Roth, C.W., Louis, C., Kalush, F., Mural, R.J., Myers, E.W., Adams, M.D., Smith, H.O., Broder, S., Gardner, M.J., Fraser, C.M., Birney, E., Bork, P., Brey, P.T., Venter, J.C., Weissenbach, J., Kafatos, F.C., Collins, F.H. and Hoffman, S.L.
Abstract:	Anopheles gambiae is the principal vector of malaria, a disease that afflicts more than 500 million people and causes more than 1 million deaths each year. Tenfold shotgun sequence coverage was obtained from the PEST strain of A. gambiae and assembled into scaffolds that span 278 million base pairs. A total of 91% of the genome was organized in 303 scaffolds; the largest scaffold was 23.1 million base pairs. There was substantial genetic variation within this strain, and the apparent existence of two haplotypes of approximately equal frequency ("dual haplotypes") in a substantial fraction of the genome likely reflects the outbred nature of the PEST strain. The sequence produced a conservative inference of more than 400,000 single-nucleotide polymorphisms that showed a markedly bimodal density distribution. Analysis of the genome sequence revealed strong evidence for about 14,000 protein-encoding transcripts. Prominent expansions in specific families of proteins likely involved in cell adhesion and immunity were noted. An expressed sequence tag analysis of genes regulated by blood feeding provided insights into the physiological adaptations of a hematophagous insect.
Keywords:	Anopheles, Blood, Chromosome Inversion, Artificial Bacterial Chromosomes, Computational Biology, DNA Transposable Elements, Digestion, Enzymes, Evolution, Expressed Sequence Tags, Feeding Behavior, Gene Expression Regulation, Insect Genes, Genetic Variation, Genome, Haplotypes, Insect Proteins, Falciparum Malaria, Molecular Sequence Data, Mosquito Control, Physical Chromosome Mapping, Plasmodium Falciparum, Single Nucleotide Polymorphism, Proteome, DNA Sequence Analysis, Species Specificity, Transcription Factors, Animals, Drosophila melanogaster
Source:	Science
ISSN:	0036-8075
Publisher:	American Association for the Advancement of Science
Volume:	298
Number:	5591
Page Range:	129-149
Date:	4 October 2002
Official Publication:	https://doi.org/10.1126/science.1076181
PubMed:	View item in PubMed

Repository Staff Only: item control page