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Ultrastructural analysis of DNA complexes during transfection and intracellular transport

Item Type:Article
Title:Ultrastructural analysis of DNA complexes during transfection and intracellular transport
Creators Name:Cartier, R., Velinova, M., Lehman, C., Erdmann, B. and Reszka, R.
Abstract:We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2-1-{my}m aggregates composed of 30-50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates.
Keywords:Gene Transfer, Polyplexes, Transfection Mechanism, NLS
Source:Journal of Histochemistry & Cytochemistry
ISSN:0022-1554
Publisher:Histochemical Soc
Volume:51
Number:9
Page Range:1237-1240
Date:September 2003
Official Publication:http://www.jhc.org/cgi/content/abstract/51/9/1237
PubMed:View item in PubMed

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