Ultrastructural analysis of DNA complexes during transfection and intracellular transport

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Item Type:	Article
Title:	Ultrastructural analysis of DNA complexes during transfection and intracellular transport
Creators Name:	Cartier, R., Velinova, M., Lehman, C., Erdmann, B. and Reszka, R.
Abstract:	We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2-1-{my}m aggregates composed of 30-50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates.
Keywords:	Gene Transfer, Polyplexes, Transfection Mechanism, NLS
Source:	Journal of Histochemistry & Cytochemistry
ISSN:	0022-1554
Publisher:	Histochemical Soc
Volume:	51
Number:	9
Page Range:	1237-1240
Date:	September 2003
Official Publication:	http://www.jhc.org/cgi/content/abstract/51/9/1237
PubMed:	View item in PubMed

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