Helmholtz Gemeinschaft


An immortalized cell line expresses properties of activated microglial cells

Item Type:Article
Title:An immortalized cell line expresses properties of activated microglial cells
Creators Name:Bocchini, V., Mazzolla, R., Barluzzi, R., Blasi, E., Sick, P. and Kettenmann, H.
Abstract:Murine cultured microglial cells were immortalized after infection with a v-raf/v-myc recombinant retrovirus. This immortalized cell line (BV-2) shares properties with body macrophages with respect to the antigen profile, their phagocytic capacity and antimicrobial activity. BV-2 cells are not constitutively able to kill tumor cells in vitro, but acquire antitumor activity following an increase in [Ca++]i. BV-2 cells, like microglial cells, are however, distinct from peripheral macrophages by their expression of inwardly rectifying K+ channels in concert with a lack in outwardly rectifying K+ channels and the formation of spineous processes. The BV-2 cell line thus represents a suitable model for in vitro studies of activated microglial cells.
Keywords:Aspergillus Fumigatus, Candida Albicans, Cell Line, Transformed Cell Line, Central Nervous System, Colony-Forming Units Assay, Cryptococcus Neoformans, Electrophysiology, Myc Genes, Interleukin-1, Macrophages, Membrane Potentials, Mesoderm, Muramidase, Oncogene Proteins v-raf, Oncogenes, Phagocytosis, Protein-Tyrosine Kinases, Oncogenic Retroviridae Proteins, Tumor Necrosis Factor-alpha, Animals, Mice
Source:Journal of Neuroscience Research
Page Range:616-621
Date:1 April 1992
Official Publication:https://doi.org/10.1002/jnr.490310405
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library