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| Item Type: | Article |
|---|---|
| Title: | Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metal loproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis |
| Creators Name: | Petrow, P.K., Wernicke, D., Schulze-Westhoff, C., Hummel, K.M., Brauer, R., Kriegsmann, J., Gromnica-Ihle, E., Gay, R.E. and Gay, S. |
| Abstract: | Objective: To study the pattern and cell type-specificity of collagenase 3, membrane-type 1 matrix metalloproteinase (MT1-MMP), and gelatinase A mRNA expression in the synovial membrane in rheumatoid arthritis (RA). Methods: The mRNA expression of collagenase 3, MT1-MMP, and gelatinase A was characterised by northern blot analysis, reverse transcriptase-polymerase chain reaction, and in situ hybridisation. In situ hybridisation was performed in combination with the immunohistochemical detection of cell type-specific antigens. Results: Synovial membrane specimens from 19 of 21 patients with RA expressing collagenase 3 mRNA were positive for MT1-MMP and gelatinase A mRNA. In control samples from patients without destructive inflammatory joint diseases collagenase 3 mRNA was not expressed and only in two of seven cases was a coexpression of MT1-MMP and gelatinase A mRNA detected. Fibroblast-like cells of the synovial membrane were found to be the predominant source of collagenase 3, MT1-MMP, and gelatinase A mRNA expression in lining and sublining layers as well as at the synovial membrane-cartilage interface. Additionally, the expression of MT1-MMP mRNA was detected in endothelial cells. Collagenase 3 mRNA expression was found in about 5% of CD68 positive macrophages. Conclusions: Collagenase 3 mRNA is expressed simultaneously with MT1-MMP and gelatinase A mRNA in fibroblast-like cells of the synovial membrane in RA. These results suggest (a) a broad extracellular proteolytic potential of fibroblast-like cells and (b) an important role of cell surface associated procollagenase 3 activation by MT1-MMP and gelatinase A for cartilage degradation by invading fibroblast-like cells. |
| Keywords: | Collagenases, In Situ Hybridization, Matrix Metalloproteinase 13, Matrix Metalloproteinase 2, Membrane-Associated Matrix Metalloproteinases, Messenger RNA, Metalloendopeptidases, Northern Blotting, Rheumatoid Arthritis, Reverse Transcriptase Polymerase Chain Reaction, Synovial Membrane |
| Source: | Annals of the Rheumatic Diseases |
| ISSN: | 0003-4967 |
| Publisher: | British Medical Journal Publishing Group |
| Volume: | 61 |
| Number: | 5 |
| Page Range: | 391-397 |
| Date: | May 2002 |
| Official Publication: | https://doi.org/10.1136/ard.61.5.391 |
| PubMed: | View item in PubMed |
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