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| Item Type: | Article |
|---|---|
| Title: | Patterns of oxidized epitopes, but not NF-κB expression, change during atherogenesis in WHHL rabbits |
| Creators Name: | Braesen, J.H., Haekkinen, T., Malle, E., Beisiegel, U. and Ylae-Herttuala, S. |
| Abstract: | Oxidative modification of lipoproteins plays an important role in atherogenesis. We investigated a variety of different oxidatively modified epitopes (malondialdehyde (MDA)-2, hydroxynonenal (HNE)-7, peroxynitrite, hypochlorite, EO-6) in parallel and compared normal vessel wall, early and advanced atherosclerotic lesions in WHHL rabbits. Early atherosclerotic lesions showed abundant intracellular staining in macrophages for all ox-epitopes, apo B and apo E; advanced lesions showed a more prominent peri- and extracellular staining for ox-epitopes, which tended to colocalize more with apo B than apo E. Hypochlorite-modified epitopes showed intense staining in all types of lesions, followed by MDA-2. Early and advanced atherosclerotic lesions differed significantly in that early stages revealed abundant cellular positivity for EO-6 and weak staining for HNE-7 modified proteins whereas the opposite was observed in advanced lesions. Nuclear factor-{kappa}B (NF-{kappa}B) was nearly exclusively detected in macrophages with no difference between early and advanced lesions. We conclude that hypochlorite-modified epitopes are abundantly present at all stages of atherogenesis. EO-6 might be a marker for early, HNE-7 a marker for advanced lesions. Colocalization of ox-epitopes with apolipoproteins further supports that oxidation of lipoproteins is one of the key mechanisms in atherogenesis. Chronic stable expression and activation of NF-{kappa}B could be a useful target for therapeutic interventions. |
| Keywords: | Atherosclerosis, Lipoprotein Oxidation, Modified Lipoproteins, WHHL Rabbit, Pathology, Immunohistochemistry |
| Source: | Atherosclerosis |
| ISSN: | 0021-9150 |
| Publisher: | Elsevier |
| Volume: | 166 |
| Page Range: | 13-21 |
| Date: | January 2003 |
| Official Publication: | https://doi.org/10.1016/S0021-9150(02)00130-2 |
| PubMed: | View item in PubMed |
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