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| Item Type: | Article |
|---|---|
| Title: | Pharmaceutical evaluation of gas-filled microparticles as gene delivery system |
| Creators Name: | Seemann, S., Hauff, P., Schultze-Mosgau, M., Lehmann, C. and Reszka, R. |
| Abstract: | Purpose. To produce and characterize a nonviral ultrasound-controlled release system of plasmid DNA (pDNA) encapsulated in gas-filled poly(D,L-lactide-co-glycolide) microparticles (PLGA-MPs). Methods. Different cationic polymers were used to form pDNA/polymer complexes to enhance the stability of pDNA during microparticle preparation. The physico-acoustical properties of the microparticles, particle size, pDNA integrity, encapsulation efficiency and pDNA release behavior were studied in vitro. Results. The microparticles had an average particle size of around 5 μm. More than 50% of all microparticles contained a gas core, and when exposed to pulsed ultrasound as used for color Doppler imaging create a signal that yields typical color patterns (stimulated acoustic emission) as a result of the ultrasound-induced destruction of the microparticles. Thirty percent of the pDNA used was successfully encapsulated and approximately 10% of the encapsulated pDNA was released by ultrasound within 10 min. Conclusions. Plasmid DNA can be encapsulated in biodegradable gas-filled PLGA-MPs without hints for a structural disintegration. A pDNA release by ultrasound-induced microparticle-destruction could be shown in vitro. |
| Keywords: | Controlled Release, DNA, Gene Therapy, Microparticles, Poly(D,L-lactide-co-glycolide), Ultrasound |
| Source: | Pharmaceutical Research |
| ISSN: | 0724-8741 |
| Publisher: | Kluwer Academic/Plenum Publ |
| Volume: | 19 |
| Number: | 3 |
| Page Range: | 250-257 |
| Date: | 1 January 2002 |
| PubMed: | View item in PubMed |
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