Adoptive tumor therapy with T lymphocytes enriched through an IFN-gamma capture assay

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Item Type:	Article
Title:	Adoptive tumor therapy with T lymphocytes enriched through an IFN-gamma capture assay
Creators Name:	Becker, C., Pohla, H., Frankenberger, B., Schueler, T., Assenmacher, M., Schendel, D.J. and Blankenstein, T.
Abstract:	Successful adoptive T-cell therapy has been demonstrated in viral disease and selected forms of cancer. However, it is limited by the difficulty to efficiently isolate and amplify autologous tumor-reactive T-cell clones. Tetramers of major histocompatibility complex (MHC) class I and peptide have facilitated the characterization of CD8 + T cells specific for tumor-associated antigens. However, for adoptive T-cell therapy, MHC-tetramers have limitations: they require knowledge of tumor antigens, which is often not available; they select T cells with a single specificity, thereby posing risk for selection of tumor escape variants; they do not select for function, so that T cells may be anergic when isolated from cancer patients; and they do not allow the isolation of CD4 + T cells that can be essential for tumor rejection. Because interferon (IFN)-{gamma} is essential for tumor rejection, we isolated live T cells based on their IFN-γ production. IFN-{gamma} secreted by previously activated T cells is retained on the cell surface, allowing their specific isolation and expansion. We show here that IFN-{gamma} but not IFN-{gamma} T cells from tumor-immunized mice are cytolytic and mediate tumor rejection upon adoptive transfer. Importantly, tumor-specific T cells can be enriched from lymphocytes infiltrating human renal cell carcinoma by the IFN-{gamma} capture assay.
Keywords:	Adoptive Immunotherapy, Colonic Neoplasms, Cultured Tumor Cells, Fibrosarcoma, Inbred BALB C Mice, Interferon Type II, Kidney Neoplasms, Neoplasms, Renal Cell Carcinoma, T-Lymphocytes, Animals, Mice
Source:	Nature Medicine
ISSN:	1078-8956
Publisher:	Nature Publishing Group
Volume:	7
Number:	10
Page Range:	1159-1162
Date:	1 January 2001
PubMed:	View item in PubMed

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