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| Item Type: | Article |
|---|---|
| Title: | Sialyl Lewisx-liposomes as vehicles for site-directed, E-selectin-mediated drug transfer into activated endothelial cells |
| Creators Name: | Stahn, R., Grittner, C., Zeisig, R., Karsten, U., Felix, S.B. and Wenzel, K. |
| Abstract: | E-selectin, exclusively expressed on activated endothelial cells, is a potential target for site-directed delivery of agents. We and others have shown that sialyl Lewisx-liposomes (sLex-liposomes) are recognized by E-selectin. We now report an approach employing sLex-liposomes to deliver antisense oligonucleotides (AS-ODNs) directed against the adhesion molecule ICAM-1 to activated vascular endothelial cells. ICAM-I expression was analyzed at the protein level by immunofluorescence and a cell surface ELISA, and at the RNA level by RT-PCR. We have investigated two different AS-ODNs complementary to the 3′ untranslated region and the AUG translation initiation codon of ICAM-1 mRNA. Both inhibited protein expression, but did not influence the mRNA level, pointing to a hybridization of AS-ODNs with the mRNA in the cytoplasm. Our results demonstrate the feasibility of a novel approach for the delivery of agents to activated endothelial cells by glycoliposomes targeted to E-selectin. |
| Keywords: | Antisense Strategy, E-Selectin, ICAM-1, Site-Directed Delivery, sLex-Liposomes |
| Source: | Cellular and Molecular Life Sciences |
| ISSN: | 1420-682X |
| Publisher: | Birkhäuser |
| Volume: | 58 |
| Number: | 1 |
| Page Range: | 141-147 |
| Date: | 1 January 2001 |
| Official Publication: | https://doi.org/10.1007/PL00000774 |
| PubMed: | View item in PubMed |
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