A monoclonal antibody directed against an autoimmune epitope on the human beta1-adrenergic receptor recognized in idiopathic dilated cardiomyopathy

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Item Type:	Article
Title:	A monoclonal antibody directed against an autoimmune epitope on the human beta1-adrenergic receptor recognized in idiopathic dilated cardiomyopathy
Creators Name:	Mobini, R., Fu, M., Wallukat, G., Magnusson, Y., Hjalmarson, A. and Hoebeke, J.
Abstract:	A monoclonal antibody (MAb M16) was obtained by immunizing Balb/C mice with free peptide H26R, corresponding to the second extracellular loop of the human {beta}1-adrenergic receptor ({beta}1AR), against which functional autoantibodies have been detected in patients with idiopathic dilated cardiomyopathy. The MAb was found to be of IgG2b type and directed against a conformational epitope, encompassing the sequence recognized by the human autoantibodies. BIAcore measurements yielded an equilibrium constant of 6.5 x 107 M1 with an association rate constant (k(on)) of 6.5 x 104 M-1 sec- 1 and a dissociation rate constant (k(off)) of 1.0 x 10-3 sec-1. It immunoprecipitated only poorly the solubilized {beta}1AR of Sf9 cell membranes. Functionally, the MAb was capable of not only reducing the number of the maximal binding sites to the {beta}1-adrenergic receptor of transfected Sf9 cell membranes, but also of displaying a positive chronotropic effect on cultured neonatal rat cardiomyocytes. These properties, which the MAb shares with the human autoantibodies, makes it an interesting tool for passive transfer studies in mice
Keywords:	Amino Acid Sequence, Antibody Affinity, Autoantigens, Beta-1 Adrenergic Receptors, Cultured Cells, Dilated Cardiomyopathy, Epitope Mapping, Heart Rate, Hybridomas, Immunoglobulin G, Inbred BALB C Mice, Molecular Sequence Data, Monoclonal Antibodies, Myocardium, Newborn Animals, Peptides, Precipitin Tests, Spodoptera, Transfection, Wistar Rats, Animals, Mice, Rats
Source:	Hybridoma
ISSN:	0272-457X
Publisher:	Mary Ann Liebert
Volume:	19
Number:	2
Page Range:	135-142
Date:	1 April 2000
Official Publication:	https://doi.org/10.1089/02724570050031176
PubMed:	View item in PubMed

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