A C/EBP beta isoform recruits the SWI/SNF complex to activate myeloid genes

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Item Type:	Article
Title:	A C/EBP beta isoform recruits the SWI/SNF complex to activate myeloid genes
Creators Name:	Kowenz-Leutz, E. and Leutz, A.
Abstract:	The activation of many genes requires the concerted effort of two or more transcription factors. Although C/EBP beta is known to cooperate with Myb to induce transcription of the granulocyte-specific mim-1 gene, the molecular mechanism of this cooperativity is undefined. We show that the N terminus of the full-length C/EBP beta isoform, which is essential for induction of the mim-1 gene in chromatin, interacts specifically with the SWI/SNF complex. Grafting this domain onto Myb generates a chimeric activator that recruits SWI/SNF and induces mim-1 transcription in the absence of C/EBP beta. Interaction between C/EBP beta and SWI/SNF is essential for activating a subgroup of resident target genes in chromatin and may represent a major determinant of combinatorial gene regulation in eukaryotes.
Keywords:	Acetyltransferases, Adenosine Triphosphatases, Binding Sites, CCAAT-Enhancer-Binding Proteins, Cell Cycle Proteins, Cell Line, Chromatin, DNA Helicases, DNA-Binding Proteins, Drosophila Proteins, Erythroblasts, Fibroblasts, Genetic Promoter Regions, Multienzyme Complexes, Nuclear Proteins, Protein Binding, Protein Isoforms, Proteins, Proto-Oncogene Proteins c-myb, Recombinant Fusion Proteins, Saccharomyces Cerevisiae Proteins, Sequence Deletion, Trans-Activators, Transcription Factors, Transcriptional Activation, Animals
Source:	Molecular Cell
ISSN:	1097-2765
Publisher:	Cell Press
Volume:	4
Number:	5
Page Range:	735-743
Date:	November 1999
Official Publication:	https://doi.org/10.1016/S1097-2765(00)80384-6
PubMed:	View item in PubMed

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