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Isolectin B4-positive and -negative nociceptors are functionally distinct

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Item Type:Article
Title:Isolectin B4-positive and -negative nociceptors are functionally distinct
Creators Name:Stucky, C.L. and Lewin, G.R.
Abstract:Small-diameter sensory neurons that are primarily nociceptors can be divided neurochemically into two populations: isolectin B(4) (IB(4))-positive nonpeptidergic neurons, and IB(4)-negative peptidergic neurons. It has been shown that IB(4)-positive neurons depend on glial-derived neurotrophic factor (GDNF), whereas IB(4)-negative neurons depend on NGF for survival during postnatal development (Molliver et al., 1997). Furthermore, these two populations of nociceptors terminate in distinct regions of the superficial spinal cord. To date, however, no evidence exists that indicates whether these two groups of nociceptors have distinct functional roles in the process of nociception (Snider and McMahon, 1998). To search for functional differences, we performed whole-cell voltage and current-clamp recordings on acutely isolated adult mouse dorsal root ganglion neurons that were labeled with fluorescent IB(4). We found that IB(4)-positive neurons have longer-duration action potentials, higher densities of TTX-resistant sodium currents, and smaller noxious heat-activated currents than IB(4)-negative neurons. Furthermore, we show that NGF, but not GDNF, directly increases the number of neurons that respond to noxious heat. The different electrophysiological properties expressed by IB(4)-positive and -negative small neurons, including their different heat sensitivities, indicates that they may relay distinct aspects of noxious stimuli both acutely and after injury in vivo.
Keywords:Sensory Neurons, Isolectin B4, Calcitonin Gene-Related Peptide, Substance P, Patch Clamp, Pain, Electrophysiology, Animals, Mice
Source:Journal of Neuroscience
Publisher:Society for Neuroscience
Page Range:6497-6505
Date:1 August 1999
Additional Information:Copyright (c) 1999 by The Society for Neuroscience
Official Publication:https://doi.org/10.1523/JNEUROSCI.19-15-06497.1999
PubMed:View item in PubMed

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