Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Telomerase protein rather than its RNA is the target of phosphorothioate-modified oligonucleotides

[thumbnail of 357oa.pdf] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
234kB

Item Type:Article
Title:Telomerase protein rather than its RNA is the target of phosphorothioate-modified oligonucleotides
Creators Name:Matthes, E. and Lehmann, C.
Abstract:Human telomerase is a ribonucleoprotein which uses its internal RNA moiety as a template for telomeric DNA synthesis. This enzyme is up-regulated in most malignant tumors and is therefore considered as a possible cancer target. Here we examined the effects of differently modified oligomers on telomeraseactivity from HL-60 cell extracts (TRAP-ezetrade mark assay). Phosphorothioate-modified oligonucleotides (PS-ODNs) inhibited telomerase activity at subnanomolar concen-trations and proved to be more efficient than peptide nucleic acids. In contrast to all the investigated oligomers, PS-ODNs were found to bind to the protein motif of telomerase called the primer binding site but poorly to its RNA. This is suggested by kinetic investigations demonstrating a competitive interaction of PS-ODNs and TS primer at the primer binding site. The K m value of the TS primer was 10.8 nM, the K i value of a 20mer PS-ODN was 1.6 nM. When the TS primer was PS-modified a striking increase in the telomerase activity was found which correlates with the number of phosphodiesters replaced. The K m value of a completely PS-modified TS primer was 0.56 nM. Based on these results the design of chimeric ODNs is proposed consisting of a 5'-PS-modified part targeting the primer binding site and a 3'-terminus part targeting the telomerase RNA.
Keywords:Antisense Oligodeoxyribonucleotides, Competitive Binding, Cultured Tumor Cells, DNA Primers, HL-60 Cells, RNA, Structure-Activity Relationship, Telomerase, Thionucleotides
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press
Volume:27
Number:4
Page Range:1152-1158
Date:15 February 1999
Official Publication:http://nar.oxfordjournals.org/cgi/content/abstract/27/4/1152
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library