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| Item Type: | Article |
|---|---|
| Title: | Identification of human myocardial proteins separated by two-dimensonal electrophoresis using an effective sample preparation for mass spectrometry |
| Creators Name: | Otto, A., Thiede, B., Mueller, E.C., Scheler, C., Wittmann-Liebold, B. and Jungblut, P. |
| Abstract: | Peptide mass fingerprinting is a powerful tool for the identification of proteins separated by two-dimensional gel electrophoresis (2-DE). The identification of in-gel digested proteins by peptide mass fingerprinting was significantly improve in comparison to blot-digests by using a peptide-collecting device. This device allows the effective purification and concentration of enzymatic digests of low-intensity spots without expensive equipment and is described in detail. Sensitivity in the fmol range was demonstrated by unequivocal identification of bovine serum albumin after sodium dodecyl sulfate--polyacrylamide gel electrophoresis. Furthermore the high performance liquid chromatography pattern of in-gel digests indicated a 2- to 3-fold higher yield of the separated peptides. Therefore, a higher amount of the peptides was available to perform N-terminal sequencing. The identification of 16 proteins from a high-resolution 2-DE gel map of human myocardium tissue has been achieved by means of this technique. Three of these proteins were associated with changes in spot intensity with dilated cardiomyopathy. |
| Keywords: | Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry, Peptide Mass Mapping, Two-Dimensional Gel Electrophoresis, Enzymatic In-Gel Digestion, Myocardial Proteins |
| Source: | Electrophoresis |
| ISSN: | 0173-0835 |
| Publisher: | Wiley |
| Volume: | 17 |
| Number: | 10 |
| Page Range: | 1643-1650 |
| Date: | 1 October 1996 |
| Official Publication: | https://doi.org/10.1002/elps.1150171027 |
| PubMed: | View item in PubMed |
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