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| Item Type: | Article |
|---|---|
| Title: | Neurofascin induces neurites by heterophilic interactions with axonal NrCAM while NrCAM requires F11 on the axonal surface to extend neurites |
| Creators Name: | Volkmer, H., Leuschner, R., Zacharias, U. and Rathjen, F.G. |
| Abstract: | Neurofascin and NrCAM are two axon-associated transmembrane glycoproteins belonging to the L1 subgroup of the Ig superfamily. In this study, we have analyzed the interaction of both proteins using neurite outgrowth and binding assays. A neurofascin-Fc chimera was found to stimulate the outgrowth of tectal cells when immobilized on an inert surface but not as a soluble form using polylysine as substrate. Antibody blocking experiments demonstrate that neurite extension on immobilized neurofascin is mediated by NrCAM on the axonal surface. Under the reverse experimental conditions where NrCAM induces neurite extension, F11, and not neurofascin, serves as axonal receptor. Binding studies using transfected COS7 cells and immunoprecipitations reveal a direct interaction between neurofascin and NrCAM. This binding activity was mapped to the Ig domains within neurofascin. The neurofascin-NrCAM binding can be modulated by alternative splicing of specific stretches within neurofascin. These studies indicate that heterophilic interactions between Ig-like proteins implicated in axonal extension underlie a regulation by the neuron. |
| Keywords: | Avian Proteins, Axons, Binding Sites, Cell Adhesion Molecules, Contactins, COS Cells, Fetus, Gene Deletion, Gene Expression, Immunoglobulins, Membrane Glycoproteins, Mutagenesis, Nerve Growth Factors, Neural Cell Adhesion Molecules, Neurites, Neuronal Cell Adhesion Molecules, Neurons, Protein Binding, Recombinant Fusion Proteins, Retina, Superior Colliculi, Tertiary Protein Structure, Animals, Chickens |
| Source: | Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Volume: | 135 |
| Number: | 4 |
| Page Range: | 1059-1069 |
| Date: | November 1996 |
| Additional Information: | Copyright (c) 1996 by The Rockefeller University Press |
| Official Publication: | http://www.jcb.org/cgi/content/abstract/135/4/1059 |
| PubMed: | View item in PubMed |
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