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| Item Type: | Article |
|---|---|
| Title: | Antisense oligonucleotides for ICAM 1 attenuate reperfusion injury and renal failure in the rat |
| Creators Name: | Haller, H., Dragun, D., Miethke, A., Park, J.K., Weis, A., Lippoldt, A., Gross, V. and Luft, F.C. |
| Abstract: | The leukocyte adhesion molecule ICAM-1 is implicated in ischemic renal reperfusion injury. We tested the utility of an ICAM-1 antisense oligodeoxyribonucleotide (ODN) with lipofectin, six hours prior to 30 minutes of bilateral renal ischemia in the rat. We measured ICAM-1 expression by immunohistochemistry and Western blot. Our antisense ODN showed a specific ICAM-1 surface expression inhibition in vitro. We then assessed ICAM-1 expression, leukocyte infiltration, serum creatinine, serum urea concentration, and renal histology in rats subjected to renal ischemia and controls. Serum creatinine and urea concentrations 12 and 24 hours post-ischemia were increased in saline treated and reverse ODN treated rats, compared to antisense ODN treated or sham operated rats (P < 0.05). Western blotting showed decreased ICAM-1 protein in antisense ODN-treated kidneys, compared to reverse ODN treated and saline treated ischemic controls (P < 0.05). Antisense ODN also ameliorated the ischemia-induced infiltration of granulocytes and macrophages (P < 0.05), and resulted in less cortical renal damage as assessed by a quantitative pathological grading scale (P < 0.05), compared to reverse ODN or saline treatment. Thus, antisense ODN for ICAM-1 protected the kidney against ischemic renal failure. The clinical applicability of these findings extends beyond ischemic acute renal failure. |
| Keywords: | Cell Adhesion, Intercellular Adhesion Molecule-1, Kidney, Acute Kidney Failure, Liposomes, Neutrophils, Antisense Oligonucleotides, Phosphatidylethanolamines, Sprague-Dawley Rats, Reperfusion Injury, Animals, Rats |
| Source: | Kidney International |
| ISSN: | 0085-2538 |
| Publisher: | Nature Publishing Group |
| Volume: | 50 |
| Number: | 2 |
| Page Range: | 473-480 |
| Date: | August 1996 |
| Official Publication: | https://doi.org/10.1038/ki.1996.338 |
| PubMed: | View item in PubMed |
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