Ectopic expression of thyrotropin releasing hormone (TRH) receptors in liver modulates organ function to regulate blood glucose by TRH

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Item Type:	Article
Title:	Ectopic expression of thyrotropin releasing hormone (TRH) receptors in liver modulates organ function to regulate blood glucose by TRH
Creators Name:	Wolff, G., Mastrangeli, A., Heinflink, M., Falck-Pedersen, E., Gershengorn, M.C. and Crystal, R.G.
Abstract:	Maintenance of blood glucose by the liver is normally initiated by extracellular regulatory molecules such as glucagon and vasopressin triggering specific hepatocyte receptors to activate the cAMP or phosphoinositide signal transduction pathways, respectively. We now show that the normal ligand-receptor regulators of blood glucose in the liver can be bypassed using an adenovirus vector expressing the mouse pituitary thyrotropin releasing hormone receptor (TRHR) cDNA ectopically in rat liver in vivo. The ectopically expressed TRHR links to the phosphoinositide pathway, providing a means to regulate liver function with TRH, an extracellular ligand that does not normally affect hepatic function. Administration of TRH to these animals activates the phosphoinositide pathway, resulting in a sustained rise in blood glucose. It should be possible to use this general strategy to modulate the differentiated functions of target organs in a wide variety of pathologic states.
Keywords:	Adenoviridae, Blood Glucose, Cultured Cells, Feasibility Studies, Gene Transfer Techniques, Genetic Vectors, Liver, Phosphatidylinositols, Sprague-Dawley Rats, Thyrotropin-Releasing Hormone Receptors, Recombinant Fusion Proteins, Signal Transduction, Thyrotropin-Releasing Hormone, Animals, Mice, Rats
Source:	Nature Genetics
ISSN:	1061-4036
Publisher:	Nature Publishing Group
Volume:	12
Number:	3
Page Range:	274-279
Date:	March 1996
Official Publication:	https://doi.org/10.1038/ng0396-274
PubMed:	View item in PubMed

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