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| Item Type: | Preprint |
|---|---|
| Title: | Atomic structure and plasticity of the CTX-MthK complex investigated by cryo-EM, NMR, and MD simulations |
| Creators Name: | Qoraj, Denis, Mohr, Swantje, Aldakul, Yessenbek K., Sprink, Thiemo, Öster, Carl, Xiao, Taoran, Schmieder, Peter, Lange, Sascha, Utesch, Tillmann, Roderer, Daniel, Chen, Shanshuang, Sun, Han and Lange, Adam |
| Abstract: | Scorpion toxins block potassium channels, disrupting cellular excitability and causing symptoms such as pain, muscle spasms, or paralysis. Here, we use an integrated structural biology approach to uncover the binding mode of the scorpion toxin charybdotoxin (CTX) to the MthK channel, a model system for human large-conductance potassium (BK) channels. Cryo-EM defines the overall architecture of the MthK–CTX complex, while complementary solution- and solid-state NMR experiments identify key binding residues and show that toxin engagement alters the selectivity filter (SF) ion configuration without rearranging the filter itself. NMR and MD simulations further reveal an anchoring lysine residue stably inserted into the SF, while other contacts undergo fast NMR timescale dynamics. Together, these findings explain how CTX-like toxins maintain exceptionally high affinity while tolerating binding across multiple K(+) channel subtypes, paving the way for site-specific extracellular modulation. |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2026.02.20.706983v2 |
| Date: | 3 March 2026 |
| Official Publication: | https://doi.org/10.64898/2026.02.20.706983 |
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