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| Item Type: | Preprint |
|---|---|
| Title: | Towards crystal structures of filament forming proteins |
| Creators Name: | Roske, Yvette, Leidert, Martina, Rehbein, Kristina and Diehl, Anne |
| Abstract: | Filament-forming proteins such as TasA (Bacillus subtilis) and camelysins CalY1, CalY2 (Bacillus cereus) pose a particular challenge for structural analysis due to their strong tendency to self-association and their polydispersity, which severely limits their ability to crystallize or to be a target for NMR-spectroscopy. To address this, it is necessary to modify the amino acid sequence to prevent filamentation. Engineering a series of N- and C-terminal truncated variants by removing flexible parts is often key to success. N-terminal extensions are also a powerful tool for obtaining crystals of fiber-forming proteins. |
| Keywords: | Bacillus Subtilis, Bacillus Cereus, Biofilm, CalY, TasA, Structure, Filaments |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2026.02.22.707290 |
| Date: | 22 February 2026 |
| Official Publication: | https://doi.org/10.64898/2026.02.22.707290 |
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