Immunofluorescence imaging-guided laser microdissection for ultralow input spatial tissue proteomics

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Item Type:	Article
Title:	Immunofluorescence imaging-guided laser microdissection for ultralow input spatial tissue proteomics
Creators Name:	Fritzsche, Sonja and Coscia, Fabian
Abstract:	Laser microdissection (LMD) enables the precise isolation of specific cells or regions of interest from tissue sections, guiding downstream molecular analyses with high spatial resolution. In this protocol, we describe an optimized protocol combining whole-slide immunofluorescence imaging, LMD, and low-input liquid chromatography (LC) mass spectrometry (MS)-based proteomics of FFPE tissue sections.
Keywords:	Laser Microdissection, Tissue Proteomics, Immunofluorescence Staining, Deep Visual Proteomics
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	Laser capture microdissection : methods and protocols
ISSN:	1064-3745
ISBN:	978-1-0716-5153-7
Publisher:	Springer / Humana Press
Volume:	3015
Page Range:	83-94
Date:	2026
Official Publication:	https://doi.org/10.1007/978-1-0716-5154-4_7
PubMed:	View item in PubMed

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