FOXO1 re-expression with a dual-recombinase allele rescues class switching in germinal center B cells

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Item Type:	Article
Title:	FOXO1 re-expression with a dual-recombinase allele rescues class switching in germinal center B cells
Creators Name:	Farré Díaz, Carlotta, Kabrani, Eleni, Winkler, Wiebke, Blanc, Eric, Wollert-Wulf, Brigitte, Salomon, Claudia, Wunderlich, F. Thomas, Beule, Dieter, Janz, Martin and Rajewsky, Klaus
Abstract:	Modeling complex (patho)physiological processes by sequential targeted mutagenesis in mice is limited by the lack of precision of cellular targeting and complex breeding strategies. We present a new Cre/DreERT2 dual-recombinase germinal center B-cell (GCBC)-specific strain, with co-expression of the recombinases from a single allele. This enables highly efficient Cre-mediated FOXO1 knockout in GCBCs in vivo, followed by time controlled, efficient Dre-mediated FOXO1 re-expression in the same cells, leading to functional rescue of GC compartmentalization and class switch recombination. The present approach can be easily adapted to other cellular contexts.
Keywords:	Germinal Center, Sequential Mutagenesis, Dual-Recombinase, Cre, Dre, Foxo1, Animals, Mice
Source:	Journal of Experimental Medicine
ISSN:	0022-1007
Publisher:	Rockefeller University Press
Volume:	223
Number:	3
Page Range:	e20241136
Date:	2 March 2026
Official Publication:	https://doi.org/10.1084/jem.20241136
PubMed:	View item in PubMed
Related to:	URL URL Type https://www.ncbi.nlm.nih.gov/sra/PRJNA1365124 External Dataset

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