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| Item Type: | Article |
|---|---|
| Title: | Remodelling of the endothelial extracellular matrix promotes smooth muscle cell hyperplasia in pulmonary hypertension due to left heart disease |
| Creators Name: | Nambiar Veetil, Netra, Gransar, Tara, Liu, Shao-Fei, Almalla, Ahed, Kirchner, Marieluise, Brackin-Helmers, Robyn, Hennig, Felix, Yeter, Ruhi, Weinhart, Marie, Mertins, Philipp, Falk, Volkmar, Szulcek, Robert, Kucherenko, Mariya M., Kuebler, Wolfgang M. and Knosalla, Christoph |
| Abstract: | AIMS: Hyperplasia of pulmonary arterial smooth muscle cells (SMCs) contributes to the progression of pulmonary hypertension (PH), yet the underlying pathomechanism of this process in PH secondary to left heart disease (PH-LHD) is poorly understood. We aimed to investigate the role of the endothelial extracellular matrix (ECM), specifically the pulmonary arterial basement membrane (BM), in influencing SMC proliferation and phenotypic changes in PH-LHD. METHODS AND RESULTS: SMC hyperplasia and endothelial ECM remodelling were characterized histologically on human pulmonary arterial samples, and by mass spectrometry, and atomic force microscopy on decellularized ECM (dECM) produced in vitro by endothelial cells isolated from pulmonary arteries (PA) of LHD patients without pulmonary hypertension (LHD w/o PH), PH-LHD patients, or healthy-heart controls. Proliferation and migration rates of SMC cultured on endothelial dECM were assessed by Ki67 immunostaining and by wound-healing assay, respectively. The role of mechanosensitive YAP1 in SMC hyperplasia was addressed in human cells and in an aortic-banding rat model of PH-LHD by analysing YAP1 activation and the effect of YAP1 inhibition. PA of LHD w/o PH and PH-LHD patients showed extensive remodelling of the BM. This was confirmed in vitro as altered composition and stiffening of dECM generated by respective patient endothelial cells. ECM remodelling was associated with SMC accumulation in the pulmonary arterial intima in patient samples and promoted SMC migration and proliferation in vitro. Conversely, dECM generated by healthy human endothelial cells reduced the hypermigration and hyperproliferation of SMC from LHD w/o PH and PH-LHD patients. Remodelling of the endothelial ECM in LHD w/o PH and PH-LHD patients also activated YAP1 in SMC, inhibition of which reduced SMC migration and proliferation in vitro. These findings were reproduced in vivo in a rat model of PH-LHD induced by aortic-banding. CONCLUSION: Here, we report endothelial ECM remodelling as a key mechanism driving SMC hyperplasia in PH-LHD. Notably, endothelial ECM remodelling is evident in both patients with LHD w/o PH and those with PH-LHD, raising the possibility that it may reflect an early event in LHD-induced pulmonary vascular remodelling. This ECM remodelling is associated with YAP1 in adjacent SMC, promoting their migration and proliferation and contributing to SMC hyperplasia. Consequently, targeting ECM remodelling and YAP1 activation may offer promising therapeutic strategies for preventing of PA remodelling in PH-LHD. |
| Keywords: | Pulmonary Hypertension, Left Heart Disease, Endothelial ECM, YAP1, Smooth Muscle Cell Hyperplasia, Animals, Rats |
| Source: | Cardiovascular Research |
| ISSN: | 0008-6363 |
| Publisher: | Oxford University Press |
| Page Range: | cvaf238 |
| Date: | 3 December 2025 |
| Official Publication: | https://doi.org/10.1093/cvr/cvaf238 |
| PubMed: | View item in PubMed |
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