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| Item Type: | Article |
|---|---|
| Title: | Fecal detection of calprotectin subunits links inflammatory bowel disease activity with chronicity of intestinal inflammation |
| Creators Name: | Jukic, Almina, Hilbe, Richard, Zundel, Luis, Willeit, Peter, Faserl, Klaus, Plattner, Christina, Zollner, Andreas, Meyer, Moritz, Siegmund, Kerstin, Klepsch, Victoria, Marteau, Valentin, Vich Vila, Arnau, Schwärzler, Julian, Vouk, Kathrin, Kozsar, Anna, Rieder, Dietmar, Weichberger, Amos, Sarg, Bettina, Grabherr, Felix, Mayr, Lisa, Moser, Patrizia, Nemati, Niloofar, Scholl-Bürgi, Sabine, Karall, Daniela, Vogel, Georg F., Welz, Lina, Aldrian, Denise, Koch, Robert, Pfister, Alexandra, Ran, Qitao, Kaser, Arthur, Blumberg, Richard S., Tancevski, Ivan, Sommer, Felix, Bacher, Petra, Schreiber, Stefan, Rosenstiel, Philip, Aden, Konrad, Baier, Gottfried, Bakiri, Latifa, Müller, Thomas, Weiss, Günter, Weersma, Rinse K., Trajanoski, Zlatko, Wagner, Erwin F., Tilg, Herbert and Adolph, Timon E. |
| Abstract: | BACKGROUND & AIMS: Quantification of the human S100A8/ S100A9 tetrameric protein complex in stool, referred to as fecal calprotectin, is an extensively validated biomarker supporting the diagnosis and management of gastrointestinal diseases. Here, we studied the quaternary protein structures (termed configuration) of S100A8 and S100A9 and their biological function in inflammatory bowel diseases (IBD). METHODS: We dissected fecal S100A8 and S100A9 configurations in patients with IBD by size-exclusion chromatography coupled with tandem mass spectrometry and systematically defined human S100A8 and S100A9 homodimer functions compared with the calprotectin heterotetramer (CP) in the intestine of mice and in human epithelium and T cells. Moreover, we report a protein interaction network of fecal S100A8 and S100A9 in IBD. RESULTS: Stool from patients with active IBD contained abundant S100A8 and S100A9 dimers besides CP. Fecal S100A9 detection associated with clinical and endoscopic disease activity in IBD patients with low CP concentration. Oral exposure to human recombinant S100A8 and S100A9 homodimers, but not to CP, worsened intestinal inflammation in toxic and genetic mouse models. Functional profiling revealed that human S100A8 and S100A9 homodimers enhanced activation of cluster of differentiation 4(+) and 8(+) T cells, which promoted experimental colitis. In turn, genetic inactivation of S100a9 protected against experimental enteritis and colitis, and pharmacologic inhibition of S100A9 ameliorated chronic colitis. CONCLUSIONS: Collectively, this study links the detection of fecal S100A9 dimers with clinical and endoscopic disease activity in IBD and identifies inflammatory actions of S100A8 and S100A9 homodimers in the intestine. Our findings pave the way for novel diagnostic and therapeutic approaches in patients with inflammatory diseases of the intestine. |
| Keywords: | Calprotectin, S100A8, S100A9, Inflammatory Bowel Diseases, Crohn's Disease, Ulcerative Colitis, Intestinal Inflammation |
| Source: | Gastroenterology |
| ISSN: | 0016-5085 |
| Publisher: | Elsevier / AGA Institute |
| Date: | 26 November 2025 |
| Additional Information: | Ashley Sanders and Leif S. Ludwig are members of the TRR241 IBDome Consortium. - ProteomeXchange dataset PXD063879 has been reserved by the PRIDE repository for a dataset that has been deposited, but is not yet publicly released and announced to ProteomeXchange. |
| Official Publication: | https://doi.org/10.1053/j.gastro.2025.08.040 |
| PubMed: | View item in PubMed |
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