Phosphoprofile reorganization of the actin binding protein Drebrin during long term depression

Tools

Item Type:	Dataset
Title:	Phosphoprofile reorganization of the actin binding protein Drebrin during long term depression
Creators Name:	Kirchner, Marieluise and Mertins, Philipp
Abstract:	Drebrin (DBN), an actin-binding protein critical for the structural integrity and function of dendritic spines, is highly phosphorylated at steady state in neurons. Here, we investigate the phosphorylation dynamics of DBN in the context of chemically induced long-term depression (cLTD), a synaptic plasticity model mimicking activity-dependent weakening of synapses. Using biochemical analyses and mass spectrometry analyses, we show that DBN undergoes rapid and robust changes in phosphorylation following cLTD induction. Notably, cLTD triggers a marked decrease in many DBN phosphorylation sites, accompanied by proteolytic cleavage of the protein, suggesting a tightly regulated mechanism linking post-translational modification to structural remodelling of the synapse. Our findings highlight the dynamic regulation of DBN by phosphorylation during synaptic depression and support its potential role as a modulator of activity-dependent synaptic plasticity.
Source:	PRIDE
Publisher:	EMBL-EBI
Date:	17 October 2025
External Fulltext:	View full text on external repository or document server
Related to:	URL URL Type https://edoc.mdc-berlin.de/25931/ Publication

Repository Staff Only: item control page