![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Preprint)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB |
![[thumbnail of Supplementary Material]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplementary Material)
6MB |
| Item Type: | Preprint |
|---|---|
| Title: | Evolutionarily conserved transcriptional regulators control monoaminergic neuron development |
| Creators Name: | Lewis, Clifton, Goulty, Matthew, Wroblewska, Aniela, Croxall, Nicola, Onion, David, Robinson, Sue, Zinzen, Robert P., Solana, Jordi, Kyriacou, Charalambos P., Rosato, Ezio and Feuda, Roberto |
| Abstract: | To what extent conserved developmental programs specify homologous cell types is a central question in biology. Here, we address this by focusing on reconstructing monoaminergic neuron development in Drosophila melanogaster embryo using time-resolved single-cell genomics, spatial transcript mapping with hybridisation chain reaction, and targeted metabolomics. We uncover a regulatory landscape in which specific transcription factors are activated before biosynthetic enzymes, establishing a prospective temporal architecture for monoaminergic fate specification. Comparative analyses of developmental single-cell atlases from zebrafish and sea urchin indicate that components of this machinery are conserved across ~550 million years of bilaterian evolution with orthologous transcription factors showing similar temporal dynamics. Together, these findings point to a putatively conserved regulatory core that interfaces with other context-dependent transcription factors; this interplay accommodates monoaminergic multifunction and subtype diversity across distinct neuroanatomies. |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2025.10.29.685200v2 |
| Date: | 31 October 2025 |
| Official Publication: | https://doi.org/10.1101/2025.10.29.685200 |
| Related to: |
Repository Staff Only: item control page
