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| Item Type: | Article |
|---|---|
| Title: | Multicolor fate mapping of microglia reveals polyclonal proliferation, heterogeneity, and cell-cell interactions after ischemic stroke in mice |
| Creators Name: | Kikhia, Majed, Schilling, Simone, Herzog, Marie-Louise, Livne, Michelle, Semtner, Marcus, Tay, Tuan Leng, Prinz, Marco, Kettenmann, Helmut, Endres, Matthias, Kronenberg, Golo, Göttert, Ria and Gertz, Karen |
| Abstract: | Microglial proliferation is a principal element of the inflammatory response to brain ischemia. However, the precise proliferation dynamics, phenotype acquisition, and functional consequences of newly emerging microglia are not yet understood. Using multicolor fate mapping and computational methods, we here demonstrate that microglia exhibit polyclonal proliferation in the ischemic lesion of female mice. The peak number of clones occurs at 14 days, while the largest clones are observed at 4 weeks post-stroke. Whole-cell patch-clamp recordings of microglia reveal a homogeneous acute response to ischemia with a pattern of outward and inward currents that evolves over time. In the resolution phase, 8 weeks post-stroke, microglial cells within one clone share similar membrane properties, while neighboring microglia from different clones display more heterogeneous electrophysiological profiles. Super-resolution microscopy and live-cell imaging unmask various forms of cell-cell interactions between microglial cells from different clones. Overall, this study demonstrates the polyclonal proliferation of microglia after cerebral ischemia and suggests that clonality contributes to their functional heterogeneity. Thus, targeting clones with specific functional phenotypes may have potential for future therapeutic modulation of microglia after stroke. |
| Keywords: | Animal Disease Models, Brain Ischemia, Cell Communication, Cell Proliferation, Inbred C57BL Mice, Ischemic Stroke, Microglia, Patch-Clamp Techniques, Stroke, Animals, Mice |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 16 |
| Number: | 1 |
| Page Range: | 8294 |
| Date: | 16 September 2025 |
| Official Publication: | https://doi.org/10.1038/s41467-025-63949-3 |
| PubMed: | View item in PubMed |
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