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| Item Type: | Dataset |
|---|---|
| Title: | Dataset: rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome |
| Creators Name: | Boehm, Volker, Wallmeroth, Damaris, Wulf, Paul O., Popp, Oliver, Teixeira Alves, Luiz Gustavo, Reinecke, Lucie, Riedel, Maximilian, Wyler, Emanuel, Franitza, Marek, Becker, Kerstin, Polkovnychenko, Karina, Del Giudice, Simone, Benlasfer, Nouhad, Mertins, Philipp, Landthaler, Markus and Gehring, Niels H. |
| Abstract: | This dataset contains the imaging source data for the manuscript titled "Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome" by Boehm et al. published in Molecular Cell in 2025. The unprocessed and uncompressed imaging data (western blot and RT-PCR gels) for each main and supplemental figure can be found in the respective figure folder. For RT-PCR images, TIF (.tif) and raw Bio-Rad Image Lab Image Document (.scn) files are provided. For Western blots, multi-page TIFs (pages: 1. overlay, 2. raw chemiluminescence, 3. marker image, 4. final) are provided. Markers for western blots: PageRuler Prestained Protein Ladder (ThermoFisher Cat# 26617) and for RT-PCR: HyperLadder 1kb (Meridian Cat# BIO-33026). The PDF file "Boehm et al Overview" provides an overview of all main and supplemental figure images used in this study. |
| Source: | Mendeley Data |
| Publisher: | Elsevier |
| Date: | 31 August 2025 |
| Official Publication: | https://doi.org/10.17632/t9vzn5vwsz.1 |
| Related to: |
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