Selective depletion of cancer cells with extrachromosomal DNA via lentiviral infection

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Item Type:	Article
Title:	Selective depletion of cancer cells with extrachromosomal DNA via lentiviral infection
Creators Name:	Yi, Eunhee, Gujar, Amit D., Zhao, Dacheng, Suina, Kentaro, Jin, Xue, Pardon, Katharina, Yu, Qinghao, Kagermazova, Larisa, Korsah, Emmanuel E., Dusseau, Noah A., Boeke, Jef D., Henssen, Anton G. and Verhaak, Roel G.W.
Abstract:	Extrachromosomal DNA (ecDNA), a major focal oncogene amplification mode found across cancer, has recently regained attention as an emerging cancer hallmark, with a pervasive presence across cancers. With technical advancements such as high-coverage sequencing and live-cell genome imaging, we can now investigate the behaviors and functions of ecDNA. However, we still lack an understanding of how to eliminate ecDNA. We observed depletion of cells containing ecDNA during lentiviral but not transposon-based transduction while we sought to investigate the mechanism of ecDNA behavior. This discovery may provide critical information on utilizing a lentiviral system in emerging ecDNA research. Additionally, this observation suggests specific sensitivities for cells with ecDNA.
Keywords:	Extrachromosomal DNA, Focal Oncogene Amplification, Lentiviral Infection
Source:	Cancer Research Communications
ISSN:	2767-9764
Publisher:	American Association for Cancer Research
Volume:	5
Number:	8
Page Range:	1458-1465
Date:	August 2025
Official Publication:	https://doi.org/10.1158/2767-9764.crc-25-0144
PubMed:	View item in PubMed

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