![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Dataset |
|---|---|
| Title: | Multicenter longitudinal quality assessment of MS-based proteomics in plasma and serum |
| Creators Name: | Kardell, Oliver, Gronauer, Thomas, von Toerne, Christine, Merl-Pham, Juliane, König, Ann-Christine, Barth, Teresa K., Mergner, Julia, Ludwig, Christina, Tüshaus, Johanna, Giesbertz, Pieter, Breimann, Stephan, Schweizer, Lisa, Müller, Torsten, Kliewer, Georg, Distler, Ute, Gomez-Zepeda, David, Popp, Oliver, Qin, Di, Teupser, Daniel, Cox, Jürgen, Imhof, Axel, Küster, Bernhard, Lichtenthaler, Stefan F., Krijgsveld, Jeroen, Tenzer, Stefan, Mertins, Philipp, Coscia, Fabian and Hauck, Stefanie M. |
| Abstract: | Advancing MS-based proteomics toward clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for clinical specimens. Steps along the method design involve the determination and optimization of several bioanalytical parameters such as selectivity, sensitivity, accuracy, and precision. In a joint effort, eight proteomics laboratories belonging to the MSCoreSys initiative including the CLINSPECT-M, MSTARS, DIASyM, and SMART-CARE consortia performed a longitudinal round-robin study to assess the analysis performance of plasma and serum as clinically relevant samples. A variety of LC-MS/MS setups including mass spectrometer models from ThermoFisher and Bruker as well as LC systems from ThermoFisher, Evosep, and Waters Corporation were used in this study. As key performance indicators, sensitivity, precision, and reproducibility were monitored over time. Protein identifications range between 300 and 400 IDs across different state-of-the-art MS instruments, with timsTOF Pro, Orbitrap Exploris 480, and Q Exactive HF-X being among the top performers. Overall, 71 proteins are reproducibly detectable in all setups in both serum and plasma samples, and 22 of these proteins are FDA-approved biomarkers, which are reproducibly quantified (CV < 20% with label-free quantification). In total, the round-robin study highlights a promising baseline for bringing MS-based measurements of serum and plasma samples closer to clinical utility. |
| Keywords: | several bioanalytical parameters, q exactive hf, protein identifications range, orbitrap exploris 480, mscoresys initiative including, method design involve, free quantification, care consortia performed, clinically relevant samples, key performance indicators, art ms instruments, plasma samples closer, robin study highlights, serum advancing ms, plasma samples, robin study, analysis performance, bringing ms, waters corporation, top performers, timstof pro, steps along, reproducibly quantified, reproducibly detectable, purpose workflows, promising baseline, joint effort, clinical utility, clinical specimens, based proteomics, based measurements, approved biomarkers |
| Source: | Figshare |
| Publisher: | Digital Science |
| Date: | 7 February 2025 |
| Official Publication: | https://doi.org/10.1021/acs.jproteome.4c00644.s003 |
| Related to: |
Repository Staff Only: item control page
