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| Item Type: | Preprint |
|---|---|
| Title: | PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities |
| Creators Name: | Fink, R., Imai, S., Gockel, N., Lauer, G., Renken, K., Wietek, J., Lamothe-Molina, P.J., Fuhrmann, F., Mittag, M., Ziebarth, T., Canziani, A., Kubitschke, M., Kistmacher, V., Kretschmer, A., Sebastian, E., Schmitz, D., Terai, T., Gründemann, J., Hassan, S., Patriarchi, T., Reiner, A., Fuhrmann, M., Campbell, R.E. and Masseck, O.A. |
| Abstract: | Genetically encoded calcium (Ca(2+)) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio of red-shifted GECIs have posed significant challenges. More critically, several red-fluorescent GECIs exhibit photoswitching when exposed to blue light, thereby limiting their applicability in alloptical experimental approaches. Here, we present the development of PinkyCaMP, the first mScarlet-based Ca(2+) sensor that outperforms current red fluorescent sensors in brightness, photostability, signal-to-noise ratio, and compatibility with optogenetics and neurotransmitter imaging. PinkyCaMP is well-tolerated by neurons, showing no toxicity or aggregation, both in vitro and in vivo. All imaging approaches, including single-photon excitation methods such as fiber photometry, widefield imaging, miniscope imaging, as well as two-photon imaging in awake mice, are fully compatible with PinkyCaMP. |
| Keywords: | Animals, Mice |
| Source: | bioRxiv |
| ISSN: | 2692-8205 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2024.12.16.628673 |
| Date: | 17 December 2024 |
| Official Publication: | https://doi.org/10.1101/2024.12.16.628673 |
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