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| Item Type: | Article |
|---|---|
| Title: | Nuclear microRNA 9 mediates G-quadruplex formation and 3D genome organization during TGF-β-induced transcription |
| Creators Name: | Cordero, J., Swaminathan, G., Rogel-Ayala, D.G., Rubio, K., Elsherbiny, A., Mahmood, S., Szymanski, W., Graumann, J., Braun, T., Günther, S., Dobreva, G. and Barreto, G. |
| Abstract: | The dynamics of three-dimensional (3D) genome organization are essential to transcriptional regulation. While enhancers regulate spatiotemporal gene expression, chromatin looping is a means for enhancer-promoter interactions yielding cell-type-specific gene expression. Further, non-canonical DNA secondary structures, such as G-quadruplexes (G4s), are related to increased gene expression. However, the role of G4s in promoter-distal regulatory elements, such as super-enhancers (SE), and in chromatin looping has remained elusive. Here we show that mature microRNA 9 (miR-9) is enriched at promoters and SE of genes that are inducible by transforming growth factor beta 1 (TGFB1) signaling. Moreover, we find that miR-9 is required for formation of G4s, promoter-super-enhancer looping and broad domains of the euchromatin histone mark H3K4me3 at TGFB1-responsive genes. Our study places miR-9 in the same functional context with G4s and promoter-enhancer interactions during 3D genome organization and transcriptional activation induced by TGFB1 signaling, a critical signaling pathway in cancer and fibrosis. |
| Keywords: | Cell Nucleus, Chromatin, G-Quadruplexes, Gene Expression Regulation, Genetic Enhancer Elements, Genetic Promoter Regions, Genetic Transcription, Histones, Human Genome, MicroRNAs, Signal Transduction, Transcriptional Activation, Transforming Growth Factor beta1, Animals |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 15 |
| Number: | 1 |
| Page Range: | 10711 |
| Date: | 20 December 2024 |
| Official Publication: | https://doi.org/10.1038/s41467-024-54740-x |
| PubMed: | View item in PubMed |
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