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| Item Type: | Article |
|---|---|
| Title: | Protocol for high-resolution 3D spatial transcriptomics using Open-ST |
| Creators Name: | Schott, M., León-Periñán, D., Splendiani, E., Ferretti, E., Macino, G., Karaiskos, N. and Rajewsky, N. |
| Abstract: | Spatial transcriptomics (ST) is fundamental for understanding molecular mechanisms in health and disease. Here, we present a protocol for efficient and high-resolution ST in 2D/3D with Open-ST. We describe all steps for repurposing Illumina flow cells into spatially barcoded capture areas and preparing ST libraries from stained cryosections. We detail the computational workflow for generating 2D/3D molecular maps (“virtual tissue blocks”), aligned with histological data, unlocking molecular pathways in space. Open-ST is applicable to any tissue, including clinical samples. For complete details on the use and execution of this protocol, please refer to Schott et al. |
| Source: | STAR Protocols |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 6 |
| Number: | 1 |
| Page Range: | 103521 |
| Number of Pages: | 1 |
| Date: | 21 March 2025 |
| Official Publication: | https://doi.org/10.1016/j.xpro.2024.103521 |
| PubMed: | View item in PubMed |
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