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| Item Type: | Article |
|---|---|
| Title: | The role of glycocalyx diversity and thickness for nanoparticle internalization in M1-/M2-like macrophages |
| Creators Name: | Liu, Y., He, Y., Xu, H., Remmo, A., Wiekhorst, F., Heymann, F., Liu, H., Schellenberger, E., Häckel, A., Hauptmann, R., Taupitz, M., Shen, Y., Yilmaz, E.Y., Müller, D.N., Heidemann, L., Schmidt, R. and Savic, L.J. |
| Abstract: | Very small superparamagnetic iron oxide nanoparticles (VSOPs) show diagnostic value in multiple diseases as a promising MRI contrast agent. Macrophages predominantly ingest VSOPs, but the mechanism remains unclear. This study identifies differences in VSOP uptake between pro-inflammatory M1 and anti-inflammatory M2 macrophages and explores the role of the pericellular glycocalyx. Glycosaminoglycans (GAG) synthesis activities and the pericellular glycocalyx for M1/M2-like macrophages were assessed by RT-qPCR, Click-iT reaction, and WGA-FITC staining. The uptake of europium-VSOP and Synomag by the two subtypes was measured using Prussian blue staining, fluorescent microscopy, and magnetic particle spectroscopy. The findings revealed that M2-like macrophages had higher GAG synthesis activity, a thicker glycocalyx, and increased nanoparticle uptake compared to M1-like macrophages. Enzymatic glycocalyx degradation significantly decreased nanoparticle uptake. This study demonstrates a positive correlation between glycocalyx and nanoparticle uptake that could be exploited for imaging and targeted therapy, particularly in cancer, where macrophage subtypes play distinct roles. |
| Keywords: | M1/M2 Macrophages, Glycocalyx, Nanoparticles Uptake, VSOP, SPION, Animals, Mice |
| Source: | Nano Letters |
| ISSN: | 1530-6992 |
| Publisher: | American Chemical Society (ACS) |
| Volume: | 24 |
| Number: | 49 |
| Page Range: | 15607-15614 |
| Date: | 11 December 2024 |
| Official Publication: | https://doi.org/10.1021/acs.nanolett.4c04004 |
| PubMed: | View item in PubMed |
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