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Transcriptomic mapping of the 5-HT receptor landscape

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Item Type:Article
Title:Transcriptomic mapping of the 5-HT receptor landscape
Creators Name:de Filippo, R. and Schmitz, D.
Abstract:Serotonin (5-HT) is crucial for regulating brain functions such as mood, sleep, and cognition. This study presents a comprehensive transcriptomic analysis of 5-HT receptors (Htrs) across ≈4 million cells in the adult mouse brain using single-cell RNA sequencing (scRNA-seq) data from the Allen Institute. We observed differential transcription patterns of all 14 Htr subtypes, revealing diverse prevalence and distribution across cell classes. Remarkably, we found that 65.84% of cells transcribe RNA of at least one Htr, with frequent co-transcription of multiple Htrs, underscoring the complexity of the 5-HT system even at the single-cell dimension. Leveraging a multiplexed error-robust fluorescence in situ hybridization (MERFISH) dataset provided by Harvard University of ≈10 million cells, we analyzed the spatial distribution of each Htr, confirming previous findings and uncovering novel transcription patterns. To aid in exploring Htr transcription, we provide an online interactive visualizer.
Keywords:5-HT Receptors, Transcriptomics, 5-HT, Serotonin, scRNA-seq, MERFISH, Animals, Mice
Source:Patterns
ISSN:2666-3899
Publisher:Cell Press / Elsevier
Page Range:101048
Number of Pages:1
Date:29 August 2024
Official Publication:https://doi.org/10.1016/j.patter.2024.101048

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