TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation

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Item Type:	Article
Title:	TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation
Creators Name:	Lillemeier, B.F., Mörtelmaier, M.A., Forstner, M.B., Huppa, J.B., Groves, J.T. and Davis, M.M.
Abstract:	The organization and dynamics of receptors and other molecules in the plasma membrane are not well understood. Here we analyzed the spatio-temporal dynamics of T cell antigen receptor (TCR) complexes and linker for activation of T cells (Lat), a key adaptor molecule in the TCR signaling pathway, in T cell membranes using high-speed photoactivated localization microscopy, dual-color fluorescence cross-correlation spectroscopy and transmission electron microscopy. In quiescent T cells, both molecules existed in separate membrane domains (protein islands), and these domains concatenated after T cell activation. These concatemers were identical to signaling microclusters, a prominent hallmark of T cell activation. This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction.
Keywords:	Signal Transducing Adaptor Proteins, Cell Membrane, Genetic Vectors, Green Fluorescent Proteins, Kinetics, Lymphocyte Activation, Membrane Microdomains, Membrane Proteins, Transmission Electron Microscopy, Fluorescence Microscopy, Biological Models, Phosphoproteins, Protein Transport, T-Cell Antigen Receptors, Recombinant Fusion Proteins, Retroviridae, T-Lymphocytes, Transfection, Animals, Mice
Source:	Nature Immunology
ISSN:	1529-2908
Publisher:	Nature Publishing Group
Volume:	11
Number:	1
Page Range:	90-6
Date:	January 2010
Official Publication:	https://doi.org/10.1038/ni.1832
PubMed:	View item in PubMed

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