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| Item Type: | Article |
|---|---|
| Title: | TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation |
| Creators Name: | Lillemeier, B.F., Mörtelmaier, M.A., Forstner, M.B., Huppa, J.B., Groves, J.T. and Davis, M.M. |
| Abstract: | The organization and dynamics of receptors and other molecules in the plasma membrane are not well understood. Here we analyzed the spatio-temporal dynamics of T cell antigen receptor (TCR) complexes and linker for activation of T cells (Lat), a key adaptor molecule in the TCR signaling pathway, in T cell membranes using high-speed photoactivated localization microscopy, dual-color fluorescence cross-correlation spectroscopy and transmission electron microscopy. In quiescent T cells, both molecules existed in separate membrane domains (protein islands), and these domains concatenated after T cell activation. These concatemers were identical to signaling microclusters, a prominent hallmark of T cell activation. This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction. |
| Keywords: | Signal Transducing Adaptor Proteins, Cell Membrane, Genetic Vectors, Green Fluorescent Proteins, Kinetics, Lymphocyte Activation, Membrane Microdomains, Membrane Proteins, Transmission Electron Microscopy, Fluorescence Microscopy, Biological Models, Phosphoproteins, Protein Transport, T-Cell Antigen Receptors, Recombinant Fusion Proteins, Retroviridae, T-Lymphocytes, Transfection, Animals, Mice |
| Source: | Nature Immunology |
| ISSN: | 1529-2908 |
| Publisher: | Nature Publishing Group |
| Volume: | 11 |
| Number: | 1 |
| Page Range: | 90-96 |
| Date: | January 2010 |
| Additional Information: | Nat Immunol 11(6):543. |
| Official Publication: | https://doi.org/10.1038/ni.1832 |
| PubMed: | View item in PubMed |
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