Continuous T cell receptor signaling required for synapse maintenance and full effector potential

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Item Type:	Article
Title:	Continuous T cell receptor signaling required for synapse maintenance and full effector potential
Creators Name:	Huppa, J.B., Gleimer, M., Sumen, C. and Davis, M.M.
Abstract:	Although signals through the T cell receptor (TCR) are essential for the initiation of T helper cell activation, it is unclear what function such signals have during the prolonged T cell-antigen-presenting cell contact. Here we simultaneously tracked TCR-CD3 complex and phosphoinositide 3-kinase activity in single T cells using three-dimensional video microscopy. Despite rapid internalization of most of the TCR-CD3, TCR-dependent signaling was still evident up to 10 h after conjugate formation. Blocking this interaction caused dissolution of the synapse and proportional reductions in interleukin 2 production and cellular proliferation. Thus TCR signaling persists for hours, has a cumulative effect and is necessary for the maintenance of the immunological synapse.
Keywords:	Antigen Presentation, CD3 Complex, Calcium, Cell Communication, Lymphocyte Activation, T-Cell Antigen Receptors, Signal Transduction, Signal Transduction, Helper-Inducer T-Lymphocytes, Animals, Mice
Source:	Nature Immunology
ISSN:	1529-2908
Publisher:	Nature Publishing Group
Volume:	4
Number:	8
Page Range:	749-55
Date:	1 August 2003
Official Publication:	https://doi.org/10.1038/ni951
PubMed:	View item in PubMed

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