Rapid multiplex analysis of lipid raft components with single-cell resolution

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Item Type:	Article
Title:	Rapid multiplex analysis of lipid raft components with single-cell resolution
Creators Name:	Schatzlmaier, P., Supper, V., Göschl, L., Zwirzitz, A., Eckerstorfer, P., Ellmeier, W., Huppa, J.B. and Stockinger, H.
Abstract:	Lipid rafts, a distinct class of highly dynamic cell membrane microdomains, are integral to cell homeostasis, differentiation, and signaling. However, their quantitative examination is challenging when working with rare cells, developmentally heterogeneous cell populations, or molecules that only associate weakly with lipid rafts. We present a fast biochemical method, which is based on lipid raft components associating with the nucleus upon partial lysis during centrifugation through nonionic detergent. Requiring little starting material or effort, our protocol enabled the multidimensional flow cytometric quantitation of raft-resident proteins with single-cell resolution, thereby assessing the membrane components from a few cells in complex cell populations, as well as their dynamics resulting from cell signaling, differentiation, or genetic mutation.
Keywords:	Cell Differentiation, Flow Cytometry, Jurkat Cells, Membrane Microdomains, Membrane Proteins, Mutation, Signal Transduction
Source:	Science Signaling
ISSN:	1945-0877
Publisher:	American Association for the Advancement of Science
Volume:	8
Number:	395
Page Range:	rs11
Date:	September 2015
Official Publication:	https://doi.org/10.1126/scisignal.aac5584
PubMed:	View item in PubMed

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