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| Item Type: | Article |
|---|---|
| Title: | Monomeric TCRs drive T cell antigen recognition |
| Creators Name: | Brameshuber, M., Kellner, F., Rossboth, B.K., Ta, H., Alge, K., Sevcsik, E., Göhring, J., Axmann, M., Baumgart, F., Gascoigne, N.R.J., Davis, S.J., Stockinger, H., Schütz, G.J. and Huppa, J.B. |
| Abstract: | T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs) within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities, T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence correlation spectroscopy and Förster resonance energy transfer measurements. We found exclusively monomeric TCR-CD3 complexes driving the recognition of antigenic pMHCs, which underscores the exceptional capacity of single TCR-CD3 complexes to elicit robust intracellular signaling. |
| Keywords: | Antigen Presentation, CD3 Complex, Lymphocyte Activation, Transgenic Mice, T-Cell Antigen Receptors, T-Lymphocytes / Immunology, Animals, Mice |
| Source: | Nature Immunology |
| ISSN: | 1529-2908 |
| Publisher: | Nature Publishing Group |
| Volume: | 19 |
| Number: | 5 |
| Page Range: | 487-496 |
| Date: | May 2018 |
| Official Publication: | https://doi.org/10.1038/s41590-018-0092-4 |
| PubMed: | View item in PubMed |
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