Monomeric TCRs drive T cell antigen recognition

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Item Type:	Article
Title:	Monomeric TCRs drive T cell antigen recognition
Creators Name:	Brameshuber, M., Kellner, F., Rossboth, B.K., Ta, H., Alge, K., Sevcsik, E., Göhring, J., Axmann, M., Baumgart, F., Gascoigne, N.R.J., Davis, S.J., Stockinger, H., Schütz, G.J. and Huppa, J.B.
Abstract:	T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs) within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities, T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence correlation spectroscopy and Förster resonance energy transfer measurements. We found exclusively monomeric TCR-CD3 complexes driving the recognition of antigenic pMHCs, which underscores the exceptional capacity of single TCR-CD3 complexes to elicit robust intracellular signaling.
Keywords:	Antigen Presentation, CD3 Complex, Lymphocyte Activation, Transgenic Mice, T-Cell Antigen Receptors, T-Lymphocytes / Immunology, Animals, Mice
Source:	Nature Immunology
ISSN:	1529-2908
Publisher:	Nature Publishing Group
Volume:	19
Number:	5
Page Range:	487-496
Date:	May 2018
Official Publication:	https://doi.org/10.1038/s41590-018-0092-4
PubMed:	View item in PubMed

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