![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
6MB |
![[thumbnail of Supplementary Data]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplementary Data)
6MB |
| Item Type: | Article |
|---|---|
| Title: | Microglia undergo molecular and functional adaptations to dark and light phases in male laboratory mice |
| Creators Name: | Mattei, D., Ivanov, A., Hammer, J., Ugursu, B., Schalbetter, S., Richetto, J., Weber-Stadlbauer, U., Mueller, F., Scarborough, J., Wolf, S.A., Kettenmann, H., Wollscheid, B., Beule, D. and Meyer, U. |
| Abstract: | Microglia are increasingly recognized to contribute to brain health and disease. Preclinical studies using laboratory rodents are essential to advance our understanding of the physiological and pathophysiological functions of these cells in the central nervous system. Rodents are nocturnal animals, and they are mostly maintained in a defined light-dark cycle within animal facilities, with many laboratories investigating microglial molecular and functional profiles during the animals' light (sleep) phase. However, only a few studies have considered possible differences in microglial functions between the active and sleep phases. Based on initial evidence suggesting that microglial intrinsic clock genes can affect their phenotypes, we sought to investigate differences in transcriptional, proteotype and functional profiles of microglia between light (sleep) and dark (active) phases, and how these changes are affected in pathological models. We found marked transcriptional and proteotype differences between microglia harvested from male mice during the light or dark phase. Amongst others, these differences related to genes and proteins associated with immune responses, motility, and phagocytosis, which were reflected by functional alterations in microglial synaptic pruning and response to bacterial stimuli. Possibly accounting for such changes, we found RNA and protein regulation in SWI/SNF and NuRD chromatin remodeling complexes between light and dark phases. Importantly, we also show that the time of microglial sample collection influences the nature of microglial transcriptomic changes in a model of immune-mediated neurodevelopmental disorders. Our findings emphasize the importance of considering diurnal factors in studying microglial cells and indicate that implementing a circadian perspective is pivotal for advancing our understanding of their physiological and pathophysiological roles in brain health and disease. |
| Keywords: | Light-Dark Cycle, Diurnal Cycle, Maternal Immune Activation (MIA), Microglia, Sleep, Proteomics, Transcriptomics, Reproducibility, Animals, Mice |
| Source: | Brain, Behavior and Immunity |
| ISSN: | 0889-1591 |
| Publisher: | Academic Press |
| Volume: | 210 |
| Page Range: | 571-583 |
| Date: | August 2024 |
| Official Publication: | https://doi.org/10.1016/j.bbi.2024.07.007 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
