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Comprehensive characterization of the viscoelastic properties of bovine submaxillary mucin (BSM) hydrogels and the effect of additives

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Item Type:Article
Title:Comprehensive characterization of the viscoelastic properties of bovine submaxillary mucin (BSM) hydrogels and the effect of additives
Creators Name:Rulff, H., Schmidt, R.F., Wei, L.F., Fentker, K., Kerkhoff, Y., Mertins, P., Mall, M.A., Lauster, D. and Gradzielski, M.
Abstract:This study presents a comprehensive characterization of the viscoelastic and structural properties of bovine submaxillary mucin (BSM), which is widely used as a commercial source to conduct mucus-related research. We conducted concentration studies of BSM and examined the effects of various additives, NaCl, CaCl(2), MgCl(2), lysozyme, and DNA, on its rheological behavior. A notable connection between BSM concentration and viscoelastic properties was observed, particularly under varying ionic conditions. The rheological spectra could be well described by a fractional Kelvin-Voigt model with a minimum of model parameters. A detailed proteomics analysis provided insight into the protein, especially mucin composition within BSM, showing MUC19 as the main component. Cryo-scanning electron microscopy enabled the visualization of the porous BSM network structure. These investigations give us a more profound comprehension of the BSM properties, especially those pertaining to viscoelasticity, and how they are influenced by concentration and environmental conditions, aspects relevant to the field of mucus research.
Keywords:Cattle, Elasticity, Hydrogels, Mucins, Rheology, Submandibular Gland, Submandibular Gland, Viscosity, Animals
Source:Biomacromolecules
ISSN:1526-4602
Publisher:American Chemical Society
Volume:25
Number:7
Page Range:4014-4029
Date:8 July 2024
Official Publication:https://doi.org/10.1021/acs.biomac.4c00153
PubMed:View item in PubMed

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