CD8+ cells protect mice against reinfection with the intestinal parasite Eimeria falciformis

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Item Type:	Article
Title:	CD8+ cells protect mice against reinfection with the intestinal parasite Eimeria falciformis
Creators Name:	Pogonka, T., Schelzke, K., Stange, J., Papadakis, K., Steinfelder, S., Liesenfeld, O. and Lucius, R.
Abstract:	We investigated cellular immune responses of mice infected with the apicomplexan parasite Eimeria falciformis in order to characterise protective immune mechanisms and effector functions. Adoptive transfer experiments with mesenterial lymph node cells (MLNC) from immune donor mice were performed, and the oocyst output monitored after challenge infection. Phenotypical analysis by fluorescence cytometry and T cell proliferation assay showed that already from day four post infection E. falciformis-specific lymphocytes were present in the MLN. The frequency of parasite-specific, IFN-γ producing CD4+ and CD8+ cells increased in this period by 9.8% and 16.4%, respectively. Infection experiments with IFN-γ deficient mice revealed that IFN-γ is involved in resistance to primary and secondary infection. Transfer of total MLNC from immune donors reduced the oocyst output by 65e74%, as compared to the oocyst output of animals transferred with cells from na€ ıve donors. Transfer of CD8þ cells inhibited parasite development resulting in a reduction of oocyst numbers by 42-64%, whereas CD4+ cells showed no influence on resistance to reinfection.
Keywords:	Eimeria Falciformis, Coccidiosis, Apicomplexa, Parasites, Cellular Immunity, Protective Immunity, Animals, Mice
Source:	Microbes and Infection
ISSN:	1286-4579
Publisher:	Elsevier
Volume:	12
Number:	3
Page Range:	218-226
Date:	March 2010
Official Publication:	https://doi.org/10.1016/j.micinf.2009.12.005
PubMed:	View item in PubMed

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