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| Item Type: | Article |
|---|---|
| Title: | Pathogen-reactive T helper cell analysis in the pig |
| Creators Name: | Ebner, F., Schwiertz, P., Steinfelder, S., Pieper, R., Zentek, J., Schütze, N., Baums, C.G., Alber, G., Geldhof, P. and Hartmann, S. |
| Abstract: | There is growing interest in studying host–pathogen interactions in human-relevant large animal models such as the pig. Despite the progress in developing immunological reagents for porcine T cell research, there is an urgent need to directly assess pathogen-specific T cells—an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. Here, we established that the activation marker CD154 (CD40L), known from human and mouse studies, identifies also porcine antigen-reactive CD4(+) T lymphocytes. CD154 expression was upregulated early after antigen encounter and CD4(+)CD154(+) antigen-reactive T cells coexpressed cytokines. Antigen-induced expansion and autologous restimulation enabled a time- and dose-resolved analysis of CD154 regulation and a significantly increased resolution in phenotypic profiling of antigen-responsive cells. CD154 expression identified T cells responding to staphylococcal Enterotoxin B superantigen stimulation as well as T cells responding to the fungus Candida albicans and T cells specific for a highly prevalent intestinal parasite, the nematode Ascaris suum during acute and trickle infection. Antigen-reactive T cells were further detected after immunization of pigs with a single recombinant bacterial antigen of Streptococcus suis only. Thus, our study offers new ways to study antigen-specific T lymphocytes in the pig and their contribution to host–pathogen interactions. |
| Keywords: | Antigen-Specific, Pig, Porcine CD4 T Cell, CD154, CD40 Ligand, Ascaris Suum, Candida Albicans, Streptococcus Suis |
| Source: | Frontiers in Immunology |
| ISSN: | 1664-3224 |
| Publisher: | Frontiers Media SA |
| Volume: | 8 |
| Page Range: | 565 |
| Date: | 17 May 2017 |
| Official Publication: | https://doi.org/10.3389/fimmu.2017.00565 |
| PubMed: | View item in PubMed |
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