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| Item Type: | Preprint |
|---|---|
| Title: | In vivo sequential mutagenesis in germinal center B cells using a dual-recombinase approach: FOXO1 re-expression upon FOXO1 knockout rescues class switch recombination |
| Creators Name: | Farré Díaz, C., Kabrani, E., Winkler, W., Salomon, C., Wunderlich, F.T., Janz, M. and Rajewsky, K. |
| Abstract: | Modeling complex (patho)physiological processes by sequential mutagenesis in mice is limited by the lack of optimized genetic tools and complex breeding strategies. We present a new Cre/DreERT2 dual-recombinase germinal center B-cell (GCBC)specific strain, with co-expression of the recombinases from a single allele. This enables highly efficient Cre-mediated FOXO1 knockout followed by time-controlled, efficient Dre-mediated FOXO1 re-expression and functional rescue in GCBCs, demonstrating suitability for precise targeted sequential mutagenesis in vivo. |
| Keywords: | Germinal Center, Sequential Mutagenesis, Dual-Recombinase, Cre, Dre, Animals, Mice |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2024.05.17.592915 |
| Date: | 22 May 2024 |
| Official Publication: | https://doi.org/10.1101/2024.05.17.592915 |
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