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| Item Type: | Article |
|---|---|
| Title: | A subset of HLA-B27 molecules contains peptides much longer than nonamers |
| Creators Name: | Urban, R.G., Chicz, R.M., Lane, W.S., Strominger, J.L., Rehm, A., Kenter, M.J., UytdeHaag, F.G., Ploegh, H., Uchanska-Ziegler, B. and Ziegler, A. |
| Abstract: | An unusual monoclonal antibody (MARB4) directed against HLA-B27 that reacts with only approximately 5-20% of the cell surface HLA-B27 was used for large-scale purification of these molecules. Subsequent mass spectrometry of HLA-B27-bound peptides showed that the minor MARB4-reactive population contained peptides primarily from 900 to 4000 Da in size (approximately 8-33 amino acid residues), whereas the major HLA-B27 population contained peptides in the mass range of 900-1400 Da (approximately 8-12 amino acid residues). Thus, a subset of HLA-B27 molecules binds to peptides much longer than nonamers. Typical HLA-B27-binding peptides contain arginine in position 2. Further analysis by Edman sequencing of the pooled bound peptides revealed that the major population contained substantial amounts of arginine at positions 1 and 9 (40-50%) and exclusively arginine at position 2, as expected. The minor population of peptides also contained detectable amounts of arginine at these positions, but at the level of only approximately 10%; no marked enrichment at any position was observed. These long HLA-B27-bound peptides could represent either intermediates in the formation of nonamers or adventitiously bound peptides. Lastly, in the TAP2 mutant cell line BM36.1 transfected with HLA-B*2705, MARB4-reactive HLA-B27 molecules were absent from the cell surface, indicating that the peptide transporter was required for delivery of the long peptides. Thus, during the folding of class I heavy chains, peptides of diverse lengths are available and participating. |
| Keywords: | ATP-Binding Cassette Transporters, Amino Acid Sequence, B-Lymphocytes, Carrier Proteins, HLA-B27 Antigen, Histocompatibility Antigens Class II, Member 3 Subfamily B ATP Binding Cassette Transporter, Molecular Sequence Data, Monoclonal Antibodies, Peptides, Protein Binding, Sequence Analysis, Transfection, Transformed Cell Line, Viral Cell Transformation |
| Source: | Proceedings of the National Academy of Sciences of the United States of America |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Volume: | 91 |
| Number: | 4 |
| Page Range: | 1534-1538 |
| Date: | 15 February 1994 |
| Additional Information: | Copyright © National Academy of Sciences. |
| Official Publication: | https://doi.org/10.1073/pnas.91.4.1534 |
| External Fulltext: | View full text on external repository or document server |
| PubMed: | View item in PubMed |
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