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| Item Type: | Article |
|---|---|
| Title: | Dendritic cells interact directly with naive B lymphocytes to transfer antigen and initiate class switching in a primary T-dependent response |
| Creators Name: | Wykes, M., Pombo, A., Jenkins, C. and MacPherson, G.G. |
| Abstract: | Dendritic cells (DC) are thought to initiate Ab synthesis by activation of T cells, which then provide cytokine and cell-bound “help” to B cells. Here, we provide evidence that DC can capture and retain unprocessed Ag in vitro and in vivo, and can transfer this Ag to naive B cells to initiate a specific Ab response. The response is skewed with 4- to 13-fold higher titers of IgG than IgM, and the predominant subclasses of Ab produced in naive animals are those associated with Th2-type responses. Ag retention and the skew in class switching is a physiologic phenomenon because DC loaded with Ag in vivo and isolated 24 h later initiated a class-switched, Ag-specific Ab response in naive animals. In vitro studies confirmed that DC provide naive B cells with signals that are essential for the synthesis of class-switched Ab. Taken together, these observations show that DC have an important role in the initiation of Ab synthesis by direct interaction with B cells. |
| Keywords: | Antigens, B-Lymphocytes, Cell Communication, Dendritic Cells, Fluorescein-5-isothiocyanate, Hemocyanins, Horseradish Peroxidase, Immunoglobulin Class Switching, Immunoglobulin G, Inbred Strains Rats, Interphase, Serum Albumin, T-Lymphocytes, Animals, Rats |
| Source: | Journal of Immunology |
| ISSN: | 0022-1767 |
| Publisher: | American Association of Immunologists |
| Volume: | 161 |
| Number: | 3 |
| Page Range: | 1313-1319 |
| Date: | August 1998 |
| Official Publication: | https://doi.org/10.4049/jimmunol.161.3.1313 |
| PubMed: | View item in PubMed |
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