Dendritic cells interact directly with naive B lymphocytes to transfer antigen and initiate class switching in a primary T-dependent response

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Item Type:	Article
Title:	Dendritic cells interact directly with naive B lymphocytes to transfer antigen and initiate class switching in a primary T-dependent response
Creators Name:	Wykes, M., Pombo, A., Jenkins, C. and MacPherson, G.G.
Abstract:	Dendritic cells (DC) are thought to initiate Ab synthesis by activation of T cells, which then provide cytokine and cell-bound “help” to B cells. Here, we provide evidence that DC can capture and retain unprocessed Ag in vitro and in vivo, and can transfer this Ag to naive B cells to initiate a specific Ab response. The response is skewed with 4- to 13-fold higher titers of IgG than IgM, and the predominant subclasses of Ab produced in naive animals are those associated with Th2-type responses. Ag retention and the skew in class switching is a physiologic phenomenon because DC loaded with Ag in vivo and isolated 24 h later initiated a class-switched, Ag-specific Ab response in naive animals. In vitro studies confirmed that DC provide naive B cells with signals that are essential for the synthesis of class-switched Ab. Taken together, these observations show that DC have an important role in the initiation of Ab synthesis by direct interaction with B cells.
Keywords:	Antigens, B-Lymphocytes, Cell Communication, Dendritic Cells, Fluorescein-5-isothiocyanate, Hemocyanins, Horseradish Peroxidase, Immunoglobulin Class Switching, Immunoglobulin G, Inbred Strains Rats, Interphase, Serum Albumin, T-Lymphocytes, Animals, Rats
Source:	Journal of Immunology
ISSN:	0022-1767
Publisher:	American Association of Immunologists
Volume:	161
Number:	3
Page Range:	1313-1319
Date:	August 1998
Official Publication:	https://doi.org/10.4049/jimmunol.161.3.1313
PubMed:	View item in PubMed

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