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| Item Type: | Article |
|---|---|
| Title: | Phenotypic analysis of peripheral CD4+ CD8+ T cells in the rat |
| Creators Name: | Kenny, E., Mason, D., Pombo, A. and Ramírez, F. |
| Abstract: | Among peripheral T cells, the expression of CD4 and CD8 is almost mutually exclusive. However, here we show, using flow cytometric analysis, that ex vivo approximately 6% of rat T cells stained for both CD4 and CD8. These double positive cells were also detected by confocal microscopy. Only around 50% of double positive cells expressed the CD8beta chain, the remaining cells expressed the CD8alpha chain alone. Double positive cells were blast-like with a phenotype, distinct from that of either CD4 or CD8 single positive cells, suggestive of an activated state. Previous reports of double positive T cells have also suggested that coexpression of CD4 and CD8 is linked to the activation state of the cell. There was an indication that priming animals with a hapten-carrier complex increased the ratio of CD8alphaalpha : alphabeta expressing double positive T cells, although we did not detect an increase in the frequency of double positive T cells following priming. We also show that the frequency of double positive cells was reduced following thymectomy and with age. In conclusion, these studies show that peripheral T cells expressing both CD4 and CD8 can be detected in the rat and that they are phenotypically distinct from CD4 and CD8 single positive T cells. |
| Keywords: | CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Immunophenotyping, Lymphocyte Activation, Confocal Microscopy, alpha-beta T-Cell Antigen Receptors, T-Lymphocyte Subsets, Thymus Gland / Immunology, Animals, Rats |
| Source: | Immunology |
| ISSN: | 0019-2805 |
| Publisher: | Blackwell Publishing |
| Volume: | 101 |
| Number: | 2 |
| Page Range: | 178-84 |
| Date: | October 2000 |
| Official Publication: | https://doi.org/10.1046/j.1365-2567.2000.00071.x |
| PubMed: | View item in PubMed |
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