Quantitative analysis of peptide levels and neurogenic extravasation following regeneration of afferents to appropriate and inappropriate targets

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Item Type:	Article
Title:	Quantitative analysis of peptide levels and neurogenic extravasation following regeneration of afferents to appropriate and inappropriate targets
Creators Name:	McMahon, S.B., Lewin, G.R., Anand, P., Ghatei, M.A. and Bloom, S.R.
Abstract:	We have studied quantitatively the levels of substance P and calcitonin gene-related peptide in nerves innervating skin and muscle of rats, and examined the effects of cross-anastomosing these nerves so that they regenerate to an inappropriate target. We have also compared the ability of nerves to induce neurogenic extravasation with their peptide content. Peptide was measured by radioimmunoassay in the proximal section of ligated peripheral nerves, and neurogenic oedema was measured by determination of Evans Blue extravasation induced by either systemic capsaicin treatment or topical mustard oil application. The levels of these peptides are higher in cutaneous nerves than muscle nerves. This cannot be explained by differences in the number of fibres in the nerves studied. The levels of peptides fall when cutaneous afferents reinnervate muscle, and rise when muscle afferents reinnervate skin. We suggest that these changes occur because of some tissue-specific trophic influence arising from the tissue innervated. The ability to produce extravasation in skin is highly correlated with the substance P and calcitonin gene-related peptide levels of its innervation, even when this occurs in inappropriate nerves which do not normally produce extravasation.
Keywords:	Afferent Neurons, Calcitonin Gene-Related Peptide, Inbred Strains Rats, Muscles, Nerve Regeneration, Neuronal Plasticity, Skin, Substance P, Surgical Anastomosis, Animals, Rats
Source:	Neuroscience
ISSN:	0306-4522
Publisher:	Pergamon Press / IBRO
Volume:	33
Number:	1
Page Range:	67-73
Date:	1989
Official Publication:	https://doi.org/10.1016/0306-4522(89)90311-4
PubMed:	View item in PubMed

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