![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Preprint)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
5MB |
Preview |
PDF (Supplementary Material)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
7MB |
| Item Type: | Preprint |
|---|---|
| Title: | Cryosectioning-enabled super-resolution microscopy for studying nuclear architecture at the single protein level |
| Creators Name: | Stein, J., Ericsson, M., Nofal, M., Magni, L., Aufmkolk, S., McMillan, R.B., Breimann, L., Herlihy, C.P., Lee, S.D., Willemin, A., Wohlmann, J., Arguedas-Jimenez, L., Yin, P., Pombo, A., Church, G.M. and Wu, C.T. |
| Abstract: | DNA-PAINT combined with total Internal Reflec on Fluorescence (TIRF) microscopy enables the highest localiza on precisions, down to single nanometers in thin biological samples, due to TIRF’s unique method for op cal sec oning and a aining high contrast. However, most cellular targets elude the accessible TIRF range close to the cover glass and thus require alterna ve imaging condi ons, affec ng resolu on and image quality. Here, we address this limita on by applying ultrathin physical cryosec oning in combina on with DNA-PAINT. With “tomographic & kine cally-enhanced” DNAPAINT (tokPAINT), we demonstrate the imaging of nuclear proteins with sub-3 nanometer localiza on precision, advancing the quan ta ve study of nuclear organiza on within fixed cells and mouse ssues at the level of single an bodies. We believe that ultrathin sec oning combined with the versa lity and mul plexing capabili es of DNA-PAINT will be a powerful addi on to the toolbox of quan ta ve DNAbased super-resolu on microscopy in intracellular structural analyses of proteins, RNA and DNA in situ. |
| Keywords: | Animals, Mice |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2024.02.05.576943 |
| Date: | 5 February 2024 |
| Official Publication: | https://doi.org/10.1101/2024.02.05.576943 |
Repository Staff Only: item control page
